2024 Ceramide assay kit

Ceramide assay kit

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August undefined, 2024

WebThis Human Phosphatidylcholine:ceramide cholinephosphotransferase 1 (SGMS1) ELISA kit is intended for laboratory research use only and not for use in diagnostic or therapeutic procedures. The stop solution changes color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. WebThe Qubit Protein Assay Kits are designed to make protein quantification easy and accurate. Addressing overlapping concentration ranges, the two kits together are accurate for initial sample concentrations from 12.5 μg/mL to 20 mg/mL and exhibit low protein-to-protein variation.

Acid Sphingomyelinase Activity Assay Kit - Echelon …

Web1 hour ago · Here, we use antiviral assays to evaluate a synthetic ceramide derivative α-NH2-ω-N3-C6-ceramide (AKS461) and to confirm the biological activity of C6-ceramides inhibiting SARS-CoV-2. Click-labeling with a fluorophore demonstrated that AKS461 accumulates in lysosomes. WebCompare Ceramide synthase 2 (CERS2) Human qPCR Primer Pair (NM_181746) from OriGene Technologies on Biocompare.com. Welcome Guest. Sign In Register. Products. Popular Categories; Antibody Search; Biomolecules; Assay Kits; qualifications for forensic tax specialist

Ceramide kinase mediates intrinsic resistance and inferior …

WebApr 12, 2024 · Plasma insulin was determined using Ultra-Sensitive Mouse Insulin ELISA Kit (Crystal Chem, USA) and plasma alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels were determined using Infinity reagents (ThermoFisher Scientific, USA). WebCeramides have been implicated as key mediators in signaling pathways, with outcomes as diverse as cell proliferation, differentiation, growth arrest, and apoptosis. An inherited … qualifications for funeral director

How do you measure Ceramide levels? ResearchGate

WebMar 21, 2003 · After the subsequent addition of 0.25 ml of 1 m NaCl and 0.25 ml of CHCl 3, phases were separated by centrifugation at 3000 rpm (2000 × g) for 5 min. Ceramide content in the extract was determined using a radiometric diacylglycerol assay kit (Amersham Biosciences) according to the manufacturer's instructions. WebNov 1, 2001 · After the addition of 1 ml of 1 mol/l NaCl and 1 ml of CHCl 3 and further mixing, phases were separated by centrifugation at 5,000g for 2 min. Lipid extracts in the lower phase were assayed for ceramide content using a radiometric assay kit according to the manufacturer’s instructions, as DAG kinase can phosphorylate both DAG and … qualifications for food stamps in tennesseeWebCell Biolabs’ Sphingomyelin Assay Kit is a simple fluorometric assay that measures the amount of sphingomyelin present in plasma or serum, tissue homogenates, or cell … qualifications for gaming programmer

"WebJan 7, 2024 · Ceramide measurement Ceramide was measured as described previously 36. After the treatment, cells were washed with PBS and lysed using ceramide assay lysis buffer (50 mM Tris pH-7.4... " - Ceramide assay kit

Ceramide assay kit

Glucosylceramidase/GBA Assay Kit (Colorimetric)

WebPlasma ceramides are predictors of adverse cardiovascular events resulting from unstable atherosclerotic plaque. Ceramides are complex lipids that play a central role in cell … WebOct 15, 1999 · A fluorescence-based high-performance liquid chromatographic assay to determine acid ceramidase activity Acid ceramidase (N-acylsphingosine amidohydrolase) is the lysosomal enzyme required to hydrolyze the N-acyl linkage between the fatty acid and sphingosine moieties in ceramide.

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WebHuman Ceramide kinase / CERK ELISA Kit. Ceramide is activated to ceramide 1-phosphate (C1P), a sphingolipid metabolite, by Ceramide kinase/CERK. Both Ceramide … WebCeramide Synthase (CerS2) Assay Kit or CerS2 Assay Kit offers short assay time for measuring CerS activity in biological samples. Shop from Sigma-Aldrich.

WebCytotoxicity Assay. The cytotoxicity of PEG-ceramide nanomicelles against N2a cells was measured using a CCK-8 kit. Briefly, N2a cells (dissociated into single cells) were seeded in 96-well plates at a density of 3×10 3 cells per well and incubated overnight. The cells were then incubated for 24 h with varying concentrations of PEG-ceramide ... http://www.cloud-clone.com/products/SEJ459Hu.html

WebCeramide is a sphingolipid which regulates a variety of signaling pathways in eukaryotic cells. Exogenous ceramide has been shown to induce cellular apoptosis. WebThe Acid Sphingomyelinase Assay Kit is an enzyme assay that measures acid sphingomyelinase activity in biological samples through the direct hydrolysis of a fluorogenic substrate. Assay sensitivity: 25 pmol Sample …

WebThis ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of the targets. This ELISA kit for research use only, not for therapeutic or diagnostic …

WebThe CerS Assay Kit provides an easy to use assay for measuring CerS activity in biological samples. This Kit offers the advantage of a short assay time requiring only small … qualifications for gastric bandWebThis kit recognizes human BDNF protein and is cross-reactive to mouse and rat BDNF protein. ASSAY SPECIFICITY This kit recognizes both native and recombinant human BDNF protein in serum, plasma, and cell culture supernatant samples only. Cell and tissue extract samples have not been tested with this kit. CROSS REACTIVITY qualifications for geek squadWebSep 15, 2003 · This new ceramide kinase assay is more specific than the commonly used diacylglycerol kinase method because the ubiquitous lipid diacylglycerol, the preferred substrate for diacyglycerol kinase which is usually present at higher concentrations than ceramide and can interfere with ceramide phosphorylation, is completely inactive with … qualifications for gastric surgeryWebThe CerS Assay Kit provides an easy to use assay for measuring CerS activity in biological samples. This Kit offers the advantage of a short assay time requiring only small amounts of biological material while eliminating TLC as a separation technique with its attendant degradation of NBD sphinganine. Packaging Kit contains: qualifications for gip hospiceWebThe assay will exclusively recognize both natural and recombinant human Alpha-Galactosidase A/GLA. Target information This gene encodes a homodimeric … qualifications for general contractorWebApr 13, 2024 · The literature data indicate that the cellular levels of sphingosine-1-phosphate (S1P) and ceramides (Cer) play a significant role in the pathogenesis of CRC, and the S1P/Cer ratio (described as sphingolipid rheostat) decides whether the cells will be directed towards proliferation or cellular senescence and apoptosis [ 6, 7 ]. qualifications for harp 2.0WebGW4869 is a commonly used pharmacological agent, which inhibits exosome generation. It blocks ceramide-mediated inward budding of multivesicular bodies (MVBs) and the release of mature exosomes from MVBs. [ 1] GW4869 exhibits cytotoxicity to phosphatidylserine-expressing myeloma cells. [ 2] It inhibits the secretion of IFN (interferon)-α by ... qualifications for general assembly