Bioworld ap0063
WebSep 21, 2024 · (1:500, Bioss, bs-0032R), and GAPDH (1:1000, Bioworld, AP0063) at 4°C. Then, the membranes were washed and incubated with HRP-conjugated secondary antibodies (1:10 000, Invitrogen) for 1 or 2 hoursat room tempera - ture. Immunoblots were developed using the immobilon ECL kit (Merck Millipore), and band intensities were de- http://www.antibodyreview.com/products/639024/betaActin(I102)polyclonalantibody-BioworldAntibodies-AP0060.html
Bioworld ap0063
Did you know?
WebFeb 20, 2024 · 1.2 主要试剂及仪器 胎牛血清（11011-8611）购自浙江天杭生物科技股份有限公司；青-链霉素（SV30010）和MEM 培养基（SH30024.01）均购自美国HyClone公司；LipofectamineTM2000 转染试剂盒（11668-019）购自美国Invitrogen公司；RIPA（弱）裂解液（P0013D）及ECL 均购自上海碧云天生物 ... Webrabbit anti-HSP90AA1 (Bioworld, BS1181), rabbit anti-HSPA1A (Bioworld, BS6446), rabbit anti-PIM1 (Abcam, ab75776), rabbit anti-AKT1 (Bioworld, BS1978), rabbit anti-GAPDH …
WebAP0063. GAPDH polyclonal antibody. AP0007. DDDDK-tag polyclonal antibody. AP0066. GAPDH (A531) polyclonal antibody . AP0064. Tubulin β polyclonal antibody. AP0059. ... Bioworld story Enterprise culture; … WebJun 24, 2024 · Bioworld. AP0063. MTT assay. We detected the proliferation of HO-8910 and OVCAR-3 cells by MTT assay. First, the cells were seeded to 96-well plates and incubated. After cells were digested by trypsin, complete medium was resuspended into cell suspension. The cell density was 2000 cells/well and inoculated to 96-well plates (100 …
WebWestern blot (WB) analysis of β-Actin (I102) pAb at 1:10000 dilution Lane1:HEK293T whole cell lysate Lane2:Raw264.7 whole cell lysate Lane3:H9C2 whole cell lysate WebGAPDH 37 1:3000 Rabbit Bioworld AP0063 MTT assay First, PANC-1 and SW1990 cells were trypsinized, com-pletely suspended and counted. ˙e cell density was 2000 . W et al. Cancer Cell Int P 3 10 cells/well and inoculated to 96-well plate(µ) (ning, Corning, NT, USA#)night, and the cells were repeate–s in each group. 5˚mg/ ...
Webanti-GAPDH (Bioworld, # AP0063). Following washing three times with TBST for 5 min, the plots were then incubated with horseradish peroxidase (HRP) conju-gated goat anti-rabbit IgG polyclonal secondary antibody (1:3000, Beyotime, # A0216) at room temperature for 1 h. Amersham’s ECL+ plusTM western blotting system
WebPRODUCT DATA SHEET Bioworld Technology,Inc. Bioworld Technology, Inc. Bioworld technology, co. Ltd. Add ： 1660 South Highway 100, Suite 500 St. Louis Park, Add ： … plastic canary cages for saleWebFeb 20, 2024 · The β-actin (Bioworld, BS13278) and GAPDH (Bioworld, AP0063) were used as loading controls. To study the nuclear export of FoxO3a, the nuclear and cytoplasmic total proteins were separately extracted from NRCMs using Nuclear and Cytoplasmic Protein Extraction Kit (Keygen Biotech, Jiangsu, China). plastic can holdersWebFeb 18, 2024 · GAPDH (1:1000 dilution, Bioworld, AP0063) was used as an internal control for protein loading. Protein band intensity was analyzed using ImageJ software. The phosphorylation level of YAP was ... plastic camo folding toddler chair and tableplastic candle bobechesWebLong noncoding RNA (lncRNA) plays a crucial part in all kinds of life activities, especially in myogenesis. SMARCD3 (SWI/SNF-related, matrix-associated, actin-dependent regulator of chromatin, subfamily d, member 3) is a member of the SWI/SNF protein complex and was reported to be required for cell proliferation and myoblast differentiation. In this study, we … plastic can covers lids for canned goodsWebThe articles in this collection are from BioWorld’s ongoing coverage of the COVID-19 coronavirus outbreak. Note that we have added three critical tables which are... Today's … plastic cane hsn codeWebAug 11, 2024 · Bioworld. AP0063. MTT assay. The Eca-109 and TE-1 cells were cultured to logarithmic growth phase and digested with trypsin, then cells (2000 cells/well) were re-suspended and inoculated to 96-well plates (100 µL/well) (Corning, Corning, NT, USA, Cat. # 3599) overnight. 20 µL MTT (5 mg/mL) (3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl ... plastic candy apple sticks